Synthetic biology and bioelectrochemical tools for electrogenetic system engineering.
查看更多>>作者：Lawrence Joshua M；Yin Yutong；Bombelli Paolo；Scarampi Alberto；Storch Marko；Wey Laura T；Climent-Catala Alicia；PixCell IGEM Team；Baldwin Geoff S；O'Hare Danny；Howe Christopher J；Zhang Jenny Z；Ouldridge Thomas E；Ledesma-Amaro Rodrigo
- 创建日期：2024-04-11
- 发布日期：2024-04-11
- 最新更新日期：2022-10-01
简介：
查看更多>>Synthetic biology research and its industrial applications rely on deterministic spatiotemporal control of gene expression. Recently, electrochemical control of gene expression has been demonstrated in electrogenetic systems (redox-responsive promoters used alongside redox inducers and electrodes), allowing for the direct integration of electronics with biological processes. However, the use of electrogenetic systems is limited by poor activity, tunability, and standardization. In this work, we developed a strong, unidirectional, redox-responsive promoter before deriving a mutant promoter library with a spectrum of strengths. We constructed genetic circuits with these parts and demonstrated their activation by multiple classes of redox molecules. Last, we demonstrated electrochemical activation of gene expression under aerobic conditions using a novel, modular bioelectrochemical device. These genetic and electrochemical tools facilitate the design and improve the performance of electrogenetic systems. Furthermore, the genetic design strategies used can be applied to other redox-responsive promoters to further expand the available tools for electrogenetics.
DOI10.17863/cam.85208
